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pmegfp n1 backbone  (Addgene inc)


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    Structured Review

    Addgene inc pmegfp n1 backbone
    Pmegfp N1 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmegfp+n1+backbone/mEGFP-N1+(Plasmid+%2354767)/pm41740683-172-17-22
    Average 95 stars, based on 75 article reviews
    pmegfp n1 backbone - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: Refined affinity purification protocol for CAMs using minimal mouse brain material.
    Article Snippet: The plasmid encoding the N-terminally GFP-tagged full ECD of mouse Teneurin-3 (residues 312–2715, excluding splice insert A or B) was created by subcloning the full ECD (Biocat) into the pUPE106.03 GFP vector containing a N-terminal cystatin secretion signal and Nterminal His6 tag (U-Protein Express) using BamH1 and NotI cloning sites. .. Full-length mouse Lphn2 (clone BC172691, Biocat GmbH) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the NheI and BamHI restriction sites. .. Full-length mouse Teneurin-1 (Biocat, catalog number BC138861, name pCR-XL-mODZ1) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the BamHI and NotI restriction sites added to the PCR primers.

    Article Title: Refined affinity purification protocol for CAMs using minimal mouse brain material.
    Article Snippet: Full-length mouse Lphn2 (clone BC172691, Biocat GmbH) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the NheI and BamHI restriction sites. .. Full-length mouse Teneurin-1 (Biocat, catalog number BC138861, name pCR-XL-mODZ1) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the BamHI and NotI restriction sites added to the PCR primers. .. Full-length mouse Teneurin-3 A0B0 was subcloned from the previously described GFP-tagged construct into the mCherry2-C1 backbone (catalog number 54563, Addgene) using the restriction sites BamHI and NotI.

    Polymerase Chain Reaction:

    Article Title: Refined affinity purification protocol for CAMs using minimal mouse brain material.
    Article Snippet: The plasmid encoding the N-terminally GFP-tagged full ECD of mouse Teneurin-3 (residues 312–2715, excluding splice insert A or B) was created by subcloning the full ECD (Biocat) into the pUPE106.03 GFP vector containing a N-terminal cystatin secretion signal and Nterminal His6 tag (U-Protein Express) using BamH1 and NotI cloning sites. .. Full-length mouse Lphn2 (clone BC172691, Biocat GmbH) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the NheI and BamHI restriction sites. .. Full-length mouse Teneurin-1 (Biocat, catalog number BC138861, name pCR-XL-mODZ1) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the BamHI and NotI restriction sites added to the PCR primers.

    Article Title: Refined affinity purification protocol for CAMs using minimal mouse brain material.
    Article Snippet: Full-length mouse Lphn2 (clone BC172691, Biocat GmbH) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the NheI and BamHI restriction sites. .. Full-length mouse Teneurin-1 (Biocat, catalog number BC138861, name pCR-XL-mODZ1) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the BamHI and NotI restriction sites added to the PCR primers. .. Full-length mouse Teneurin-3 A0B0 was subcloned from the previously described GFP-tagged construct into the mCherry2-C1 backbone (catalog number 54563, Addgene) using the restriction sites BamHI and NotI.

    Clone Assay:

    Article Title: Refined affinity purification protocol for CAMs using minimal mouse brain material.
    Article Snippet: The plasmid encoding the N-terminally GFP-tagged full ECD of mouse Teneurin-3 (residues 312–2715, excluding splice insert A or B) was created by subcloning the full ECD (Biocat) into the pUPE106.03 GFP vector containing a N-terminal cystatin secretion signal and Nterminal His6 tag (U-Protein Express) using BamH1 and NotI cloning sites. .. Full-length mouse Lphn2 (clone BC172691, Biocat GmbH) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the NheI and BamHI restriction sites. .. Full-length mouse Teneurin-1 (Biocat, catalog number BC138861, name pCR-XL-mODZ1) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the BamHI and NotI restriction sites added to the PCR primers.

    Article Title: Refined affinity purification protocol for CAMs using minimal mouse brain material.
    Article Snippet: Full-length mouse Lphn2 (clone BC172691, Biocat GmbH) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the NheI and BamHI restriction sites. .. Full-length mouse Teneurin-1 (Biocat, catalog number BC138861, name pCR-XL-mODZ1) was amplified by PCR and cloned into the pmEGFP-N1 backbone (catalog number 54767, Addgene), containing a C-terminal GFP tag by using the BamHI and NotI restriction sites added to the PCR primers. .. Full-length mouse Teneurin-3 A0B0 was subcloned from the previously described GFP-tagged construct into the mCherry2-C1 backbone (catalog number 54563, Addgene) using the restriction sites BamHI and NotI.



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    95
    Addgene inc pmegfp n1 backbone
    Pmegfp N1 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmegfp+n1+backbone/mEGFP-N1+(Plasmid+%2354767)/pm41740683-172-17-22
    Average 95 stars, based on 1 article reviews
    pmegfp n1 backbone - by Bioz Stars, 2026-10
    95/100 stars
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